Figure 4. Regulation of NALP1 levels in response to CREB modulators.

(A) TF1 cells were incubated with 50 ng/ml PMA (P) and 1 mM db-cAMP (dbA) and analysed for the presence of phospho-CREB by Western blotting. The asterisk denotes a cross-reactive protein recognized by the anti-pCREB antibody. The levels of CREB protein were also determined. Lane C, untreated control cells. (B) Cells were cultured in the presence of 10 ng/ml PMA (P), and 1 mM db-cAMP (dbA), and then analysed for the expression of NALP1 protein by Western blotting with an antiserum against an NALP1 peptide. The levels of β-tubulin were also determined to verify equal loading. (C) TF1 cell line was transiently transfected with a control vector (pCMV) or A-CREB. After 24 h of transfection, the mRNA levels of NALP1 were determined by real-time PCR. The histogram shows the means±S.D. for triplicate analyses.