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. 2004 Nov 23;384(Pt 2):295–305. doi: 10.1042/BJ20040955

Figure 3. PPARα–AMPKα interaction in vivo.

Figure 3

293 cells were transfected with expression plasmids for mPPARα (0.2 μg) and FLAG–hAMPKα2 (1.0 μg) as indicated. (A) mPPARα complexes were immunoprecipitated with anti-mPPARα antiserum, immunoprecipitates were resolved by SDS/PAGE and FLAG–hAMPKα2 complexes were characterized by Western blotting using anti-FLAG M2 monoclonal antiserum as described in the Experimental section. (B) Expression of FLAG–hAMPKα2 was monitored by Western blotting of cell lysates using anti-FLAG antisera. Kd, kDa.