Figure 9. Displacement by AICAR of endogenous nuclear AMPKα.

INS cells were cultured on glass coverslips and incubated for 4 h in the absence or presence of 0.5 mM AICAR. Following treatment, cells were fixed, permeabilized, further incubated with anti-AMPKα2 antibody and analysed by confocal microscopy as described in the Experimental section. The ratio of nuclear hAMPKα2/cytosolic hAMPKα2 was determined for 100 cells.