Table 3. DNA pol III cores.
DNA pol III cores were formed with wild-type α-subunit, wild type θ-subunit and the ε-subunit indicated. The mutation frequency (MF) is the number of colonies resistant to both chloramphenicol and 5-fluoro-2′-deoxyuridine divided by the total number of chloramphenicol-resistant colonies; values were averaged from two separate polymerization reactions (means±S.D.). The total number of colonies resistant to both chloramphenicol and 5-fluoro-2′-deoxyuridine ranged from 49 to 425. The pol/exo (exonuclease) ratio is the total fmol of 3′ termini extended divided by the total fmol of 3′ termini excised. Pol activities ranged from 11 to 22 fmol, and exonuclease activities ranged from 14 to 180 fmol.
| Polymerase | 104×MF | Increase in MF (fold) | Pol/exo ratio | Relative pol/exo ratio |
|---|---|---|---|---|
| None | 0.3 | |||
| Wild-type core | 1.6±0.15 | 1.0 | .1 | 1 |
| α-Subunit | 11.0±1.3 | 7.0 | − | − |
| ε186 core | 2.5 | 1.5 | − | − |
| MutD5 core | 9.2±0.10 | 6.0 | − | − |
| ε501 core | 1.7±0.31 | 1.0 | 0.16 | 1.6 |
| ε502 core | 1.7±0.18 | 1.1 | 0.11 | 1.1 |
| ε503 core | 3.8±0.23 | 2.5 | 0.2 | 2.0 |
| ε504 core | 4.4±0.42 | 2.8 | 0.8 | 8.0 |
| ε506 core | 4.3±0.18 | 2.8 | 0.90 | 9.0 |
| ε507 core | 4.1±2.6 | 2.5 | 0.84 | 8.4 |
| ε508 core | 5.6±0.76 | 3.5 | 0.77 | 7.7 |
| ε509 core | 2.0±0.23 | 1.3 | 0.25 | 2.5 |
| ε510 core | 4.2±1.3 | 2.8 | 0.83 | 8.3 |
| ε511 core | 3.8±0.58 | 2.5 | 0.35 | 3.5 |
| ε512 core | 3.9±0.70 | 2.5 | 0.80 | 8.0 |
| ε513 core | 3.8±1.7 | 2.5 | 0.25 | 2.5 |
| ε515 core | 3.1±0.88 | 2.0 | 0.08 | 0.80 |
| ε516 core | 3.0±0.40 | 2.0 | 0.3 | 3.0 |