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Recombinant yeasts expressing IgD4 or vector alone were grown in the presence of 50 μM of the n–6 PUFA, ADA (22:4n–6) or the n–3 PUFA, ω3-DPA (22:5n–3). The corresponding n–6 product, 22:5n–6, and the n–3 product, DHA (22:6n–3), formed by the Δ4-desaturase activity of IgD4, are indicated by arrows. The x-axis represents retention time (min) and y-axis represents degree of response.
