Table 1. Conversion of EPA into DHA in yeast.
Yeast strain YPH499 was transformed with two constructs (pYX242+pavELO and pESC-Ura+IgD4) or with the two empty vectors (pYX242+pESC-Ura), and grown in the presence of exogenous EPA. The values shown are means±S.D. of the fatty acid composition for three independent cultures. Fatty acid values correspond to the percentage of total fatty acids.
| Percentage of total fatty acids | ||
|---|---|---|
| Fatty acid | Empty vectors | pavELO+IgD4 |
| 16:0 | 16.01±1.52 | 17.78±0.36 |
| 16:1Δ9 | 30.26±0.94 | 22.70±0.68 |
| 18:0 | 5.33±1.42 | 5.84±0.23 |
| 18:1Δ9 | 22.78±2.9 | 25.56±1.77 |
| 18:2Δ9,12 | − | − |
| 18:3Δ9,12,15 | − | − |
| 18:4Δ6,9,12,15 | − | − |
| 20:4Δ8,11,14,17 | − | − |
| 20:5Δ5,8,11,14,17 | 25.62±5.9 | 21.20±1.06 |
| 22:5Δ7,10,13,16,19 | − | 3.11±0.97 |
| 22:6Δ4,7,10,13,16,19 | − | 3.81±0.35 |