Table 1. Effect of modifying agents on the LPL1 enzyme from C. gattii.
Assays for enzyme activities were performed at pH 5.0 or pH 7.0 using 1-palmitoyl lyso-PC as substrate. The final concentrations of modifiers in the assays are shown in the Table. The results are expressed as the percentage of the control values (100%), which were 37.5±1.9 (pH 5.0) and 2.9±0.5 (pH 7.0) μmol/min per mg of protein. Experiments were performed at least twice and produced similar results.
| Relative LPL activity (%) | ||||
|---|---|---|---|---|
| Metal ion/reagent | Concentration (mM) | pH… | 5.0 | 7.0 |
| Control | 100 | 100 | ||
| CaCl2 | 10 | 96 | 260 | |
| FeCl3 | 10 | 61 | 62 | |
| EGTA | 10 | 98 | 85 | |
| Dithiothreitol | 10 | 122 | 91 | |
| Triton X-100 | 0.1* | 52 | BLD | |
| Carnitine | 10 | 121 | 91 | |
| Palmitoyl carnitine | 0.5 | 10 | 157 | |
| DPPC | 0.5 | 16 | 50 | |
| Sphingomyelin | 0.5 | 79 | 71 | |
| PA | 0.5 | 76 | 34 | |
* Concentration is a percentage (w/v).