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. 2004 Nov 23;384(Pt 2):385–390. doi: 10.1042/BJ20041114

Figure 3. FPLC gel-filtration analysis.

Figure 3

FPLC gel-filtration chromatography profiles of purified recombinant avidin (A) and commercial avidin control (Belovo) (B). The recombinant avidin is seen almost completely as a single sharp peak corresponding to the tetrameric quaternary structure of avidin, whereas a clear higher molecular-mass peak (approx. 25 min) is seen in the chromatogram of control avidin. Elution times of gel-filtration standards are indicated in the chromatograms by arrows: IgG (158 kDa), 24.6 min; BSA (68 kDa), 27.8 min; ovalbumin (44 kDa), 30.1 min.