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. 2004 Nov 23;384(Pt 2):411–420. doi: 10.1042/BJ20041112

Figure 3. Characterization of the interaction between RNA and Ceg1.

Figure 3

(A) Increasing amounts of RNA were added to a 100 nM solution of the enzyme in binding buffer (50 mM Tris/HCl, pH 7.5, and 50 mM KOAc) and the emission spectrum was scanned from 310 to 440 nm. (B) A van't Hoff plot for the interaction between RNA and Ceg1 is shown. The effect of temperature on the association constant was evaluated at pH 7.0. (C) The effect of increase in ionic strength on the apparent Kd of Ceg1 for RNA was investigated. Increasing concentrations of KCl were added to the reactions to generate the desired ionic strengths.