Table 2. Thermodynamic unfolding parameters measured by equilibrium GdmCl denaturation.
The parameters ΔGou, m and Cm were determined by GdmCl denaturation and from the integration of the fluorescence intensity. The differences in Cm and ΔGou values in comparison with the free Ceg1 protein are also shown (ΔCm and ΔΔGou respectively).
| Protein | Cm (M) | ΔCm (M) | m (kJ·mol−1·M−1) | ΔGou (kJ/mol) | ΔΔGou (kJ/mol) |
|---|---|---|---|---|---|
| Ceg1 | 1.01 | 0.00 | 1.62 | 3.21 | 0.00 |
| Ceg1·GTP | 1.03 | 0.02 | 1.36 | 2.96 | −0.25 |
| Ceg1·GMP | 2.02 | 1.02 | 3.23 | 7.98 | 4.77 |
| Ceg1·RNA | 1.08 | 0.08 | 1.24 | 3.26 | 0.05 |