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. 2004 Nov 23;384(Pt 2):421–428. doi: 10.1042/BJ20040601

Figure 5. Binding of Eqt-II and the I18W, V22W, A25W mutants to SM/PC (1:1) MLVs.

Figure 5

The toxins (concentration 1.67 μM) were incubated overnight at 25 °C in the absence or presence of SM/PC (1:1) MLVs (L/T ratio 600:1). The incubation buffer was 10 mM Hepes, pH 7.5/200 mM NaCl. The SDS/PAGE gel shows the bands corresponding to the supernatants obtained after centrifugation of the samples. Lanes 3, 5, 7 and 9 correspond to the toxins incubated in the presence of MLVs, whereas lanes 2, 4, 6 and 8 correspond to the toxins incubated in the absence of MLV. Lane 1 corresponds to the molecular-mass markers. The same result was obtained in a duplicate experiment.