Western-blot analysis was performed with antibodies against PfΔFC and hFC using purified FC preparations as well as parasite and red cell lysates. (A) Lanes: 1, 2, 5 and 6, Western-blot analysis with PfΔFC antibodies. (B) Lanes: 1, 2, 5 and 6, Western-blot analysis with hFC antibodies; 1, PfΔFC; 2, partially purified PfFC (top) and hFC (bottom); 3 and 5, parasite lysate; 4 and 6, red blood cell lysate; 3 and 4, preimmune sera from mice (top) and rabbit (bottom) controls.