Figure 6. Western-blot analysis of the parasite organellar and cytosolic fractions.
Antibodies to (A) PfPP2C1, (B) PfNT1, (C) PfHsp60, (D) PfALAD and (E) PfFC were used. 1, Organellar fraction; 2, cytosolic fraction. The total parasite pellet isolated from 2 ml of culture (10% parasitaemia) was fractionated into organellar and cytosolic fractions and the entire fractions were analysed by SDS/PAGE for Western-blot analysis.