Figure 3. CHX+PMA enhances IκBα degradation in VA-13 cells.

Western blot analysis of IκBα levels in the cytoplasmic extracts of cells pre-treated for 1 h with CHX (10 μg/ml) followed by PMA (100 nM). Extracts were prepared 30 min, 2 h, 4 h or 6 h after PMA administration. GAPDH was used as a loading control.