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. 2004 Dec 7;384(Pt 3):543–549. doi: 10.1042/BJ20040714

Figure 4. Inhibition of PKC decreases PMA-mediated effects on NF-κB DNA-binding activity within the intronic enhancer as well as MnSOD protein.

Figure 4

(A) The effect of GF109203X on PMA (100 nM) responsiveness of the I2E/P7-dependent luciferase reporter gene. Results are means±S.E.M. for three independent experiments. +P≤0.0001, significant difference between PMA and non-treatment with each concentration of GF109203X; *P≤0.0001, significant difference compared with PMA alone. (B) EMSA of nuclear extracts (10 μg) with radiolabelled oligonucleotides. Cells were pre-treated with GF109203X (0–1 μM) for 1 h before PMA (100 nM) administration. Extracts were prepared 1 h after PMA treatment. (C) Western blot analysis of cytosolic extracts (100 μg) of cells pre-treated with GF109203X (0–1 μM) for 1 h before PMA (100 nM) administration. Extracts were prepared 24 h after PMA treatment. GAPDH was used as a loading control.