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. 2004 Dec 7;384(Pt 3):551–558. doi: 10.1042/BJ20031883

Table 4. Effect of TPP on the activity of various AOs.

Experiments were carried out in 25 mM Hepes/150 mM NaCl/1 mM TPP at pH 7.20 and 25 °C. For human AOs, the activity measurements were carried out at 37 °C as described by Holt et al. [49]. All measurements were carried out at [S]≪Km for various enzyme/substrate systems.

Source of AO Substrate Residual activity (kcat/Km) (%)
Bovine serum* SPM 0
Pig kidney Cadaverine 55
Soybean seedling Cadaverine 100
Human platelets* BZA, NONA 100
Human serum* Cadaverine 33

* Bovine serum, human serum and platelets were used without further purification.