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| Kd (μM) | ||
|---|---|---|
| Enzyme | FRET (MANT-ADP) | Equilibrium dialysis (ADP) |
| E. coli YjeE | 8±0.9 | 32±7.6 |
| K41A E. coli YjeE | 65±3.8 | 211±66 |
| T42A E. coli YjeE | >100 | Not determined |
| D80Q E. coli YjeE | >100 | Not determined |
| H. influenzae YjeE | 18±0.4 | Not determined |
