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. 2004 Dec 7;384(Pt 3):591–598. doi: 10.1042/BJ20040380

Figure 1. ZOL up-regulates BSP expression at both protein and mRNA levels in Saos-2 cells.

Figure 1

(A) Western-blot analysis of BSP expression in Saos-2 cells was performed using LFMb-24 mouse anti-human BSP monoclonal antibody. The cells were cultured in the presence of ZOL, concentrations ranging from 5 to 30 μM, during 48 h. Equal loading of total protein extracts (20 μg/lane) was assessed using an anti-α-tubulin antibody. (B) ZOL-induced changes in BSP mRNA levels in Saos-2 cells are given as fold-induction relative to untreated control cells. Total RNA from control and ZOL-treated cultures was analysed using real-time RT–PCR. The levels of BSP mRNA were normalized for variations in GAPDH mRNA levels. (C) Time-course study of BSP mRNA level in Saos-2 cells exposed to 20 μM ZOL for 24, 48 and 72 h. BSP mRNA levels of treated cells (+) at the different time points are represented as fold-induction relative to untreated cells (−) at 24 h and normalized for variations in GAPDH mRNA levels. Each experiment was performed three times with similar results. *P<0.005 and **P<0.0001 represent significant difference compared with control cell cultures.