Figure 6. ZOL prevents RhoA protein translocation from the cytosol to the cell membrane in Saos-2 cells.

(A) Saos-2 cells were treated with ZOL (20 μM) for the indicated times and (B) in the presence or absence of GGOH (20 μM) or FOH (20 μM) for 48 h. Cells were extracted and separated into membrane and cytosolic fractions to detect RhoA by immunoblotting as described in the Experimental section. These results are representative of three independent experiments.