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. 2004 Dec 7;384(Pt 3):591–598. doi: 10.1042/BJ20040380

Figure 7. RhoA and Rac1 silencing induce BSP mRNA expression.

Figure 7

(A) Saos-2 cells were transfected with calcium phosphate alone (cont), a siRNA targeting RhoA (RhoA), a siRNA targeting Rac1 (Rac1) or a siRNA targeting GL3 Luciferase (Luc). Cells were lysed 48 h post-transfection and analysed by immunoblotting with a specific antibody to RhoA or Rac1 to control RhoA and Rac1 protein synthesis blockade. Anti-α-tubulin antibody was used for normalization. (B) Saos-2 cells were transfected as described above and total RNA was extracted 48 h post-transfection. Steady-state levels of BSP mRNA were analysed using real-time RT–PCR and normalized for variations in GAPDH mRNA levels. BSP mRNA expression is given as fold-induction relative to control. Each experiment was performed three times with similar results. *P<0.005 represents significant difference compared with control cell cultures.