Figure 5. Determination of the oligomeric state of C-terminal-deletion mutants.

Sf-900 insect cells were infected with recombinant baculoviruses, which contain the mutated UDP-GlcNAc 2-epimerase/ManNAc kinase gene. The cytosolic fraction was applied to a gel-filtration column. Enzymic activity in the fractions was detected with the colorimetric UDP-GlcNAc 2-epimerase assay and plotted against the elution volume. H and T indicate the elution volumes corresponding to the respective hexamer or trimer of the investigated protein. Since the mutants possessed different activities, different amounts of protein were applied to the column.