Table 1. Oligonucleotide primers used for construction of deletion mutants of UDP-GlcNAc 2-epimerase/ManNAc kinase.

Primers were used for site-directed mutagenesis, mismatches with the template DNA are underlined.

Mutant	Primer sequence
Δ383–722	5′-TTCAAGAGCCATGACAGAAGAAATTCTGC-3′
Δ490–722	5′-GCTGCACTCGACCTAGTAGATACAGGAGTGG-3
Δ597–722	5′-GGAATGGCCTTGTAGAGGGAAGCAAAGAAGC-3′
Δ697–722	5′-GGATGTGGATGTATAGGTTTCAGACTTGG-3′
Δ717–722	5′-GGTTCTGGACTACTAGACCCGCAGGATCC-3′
Δ1–359	5′-GGTAAACAGTACCCTTGCTCGAGGATATATGGGG-3′