Table 2. Oligonucleotide primers used for construction of deletion mutants of UDP-GlcNAc 2-epimerase/ManNAc kinase for the yeast two-hybrid experiments.

Primers 1–14 were used for amplification of cDNAs for generation of yeast two-hybrid constructs (homologies with UDP-GlcNAc 2-epimerase/ManNAc kinase sequence are underlined).

Name	Sequence
Primer 1	5′-GTCGACGTCGACAATGGAGAAGAACGGGAATAACCGG-3′
Primer 2	5′-GCGGCCGCCTAGTGGATTGCGGGCG-3′
Primer 3	5′-GCGGCGCGCGGCCGCAATATCCTGAGAGATGTTCT-3′
Primer 4	5′-GTCGACGTCGACGAGTGCCTTGGCTGTTGATC-3′
Primer 5	5′-GTCGACGTCGACTATTCGGATGTGGCTAGGTTGA-3′
Primer 6	5′-GCGGCCGCGAGGTCCACAGAGTTCCACT-3′
Primer 7	5′-GCGGCCGCCAAAAGGATGCGGTCGTGGT-3′
Primer 8	5′-GTCGACGTCGACGGATGTCCTCAACCGCCTGAAGC-3
Primer 9	5′-GCGGCCGCGGGTTCCAAAGGCGCCAACC-3′
Primer 10	5′-GTCGACGTCGACGATGGTTCGAGTGATGCGGAAG-3′
Primer 11	5′-GCGGCCGCGCTGCCGTGGATCAGCTCGT-3′
Primer 12	5′-GTCGACGTCGACACCCGTGTGGGTGGACAACGA-3′
Primer 13	5′-GCGGCCGCGCGGCCGCCATAGTGTGGAGGATATTCA-3′
Primer 14	5′-GTCGACGTCGACAGTGGAAGGGATGTCAGTGCC-3′