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. 2024 Aug 22;10:372. doi: 10.1038/s41420-024-02128-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A ACSL4 expression was analyzed by RT-qPCR in a discovery cohort of TNBC (n = 33) sample patients and healthy tissue samples (n = 19) (mean ± SD). B Luciferase reporter assay was performed in HEK-293T cell line co-transfected with pEZX-MT06 (3’UTR ACSL4 containing or empty vector) and miRNA-449a, miRNA-449b-5p or miRNA-449c-5p mimics separately (mean ± SD) (n = 4). ACSL4 expression was analyzed by RT-qPCR (mean ± SD) (n = 3) (C) and western blot (D) after miRNAs-449 mimics transfection in MDA-MB-231 and MDA-MB-436 cell lines. ACSL4 expression was analyzed by RT-qPCR (mean ± SD) (n = 3) (E) and Western blot (F) after miRNAs-449 mimics transfection separately in MDA-MB-231 and MDA-MB-436 cell lines. **p < 0.01, ***p < 0.001, ****p < 0.0001. C plasmid: Control plasmid.