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. 2024 Aug 22;10:372. doi: 10.1038/s41420-024-02128-7

Fig. 7. MiRNAs-449 overexpression and ACSL4 knockdown inhibit ABCG2 expression and increase the intracellular accumulation of doxorubicin.

Fig. 7

ABCG2 expression was analyzed by RT-qPCR (mean ± SD) (n = 3) (A) and Western blot (B) in MDA-MB-231 and MDA-MB-231R cell lines. C ABCG2 expression was analyzed by western blot in MDA-MB-231, MDA-MB-436, and MDA-MB-231R cell lines after miRNAs-449 mimics, siACSL4#1, or siACSL4#2 transfection. Representative images (D) (×40 magnification) and quantification of mean fluorescence intensity (E) of doxorubicin intracellular accumulation after miRNAs-449 mimics, siACSL4#1, or siACSL4#2 transfection, and doxorubicin treatment (5 µM, 3 h) (mean ± SEM). Scale bar: 50 µm. *p < 0.05, ****p < 0.0001.