Table 2.
Deriving best fitted model of ‘Severity Score’ using the combinations of Ct values of different T cell markers in oro-nasopharyngeal swab RNA.
| Principle of Ct value combinations | Non-severe Group (n = 10) Mean ∆Ct/∆Ctcombinations | Severe Group (n = 9) Mean ∆Ct/∆Ctcombinations |
Significance of difference: Severe vs non-severe COVID-19 cases by T-TEST Pvalue |
|---|---|---|---|
| A. ΔCt values normalized with ACTB | |||
| T1- ACTB | 12.61 | 13 | 0.7 |
| T2- ACTB | 3.67 | 3.07 | 0.20 |
| T3- ACTB | 13.87 | 13.2 | 0.56 |
| T4- ACTB | 6.47 | 9.49 | 0.01* |
| T5- ACTB | 12.38 | 12.64 | 0.82 |
| T6- ACTB | 6.83 | 6.34 | 0.51 |
| T7- ACTB | 9.44 | 9.95 | 0.67 |
| T8- ACTB | 7.89 | 9.86 | 0.18 |
| B. Composite ΔCt values by Combination of ACTB normalized ΔCt values | |||
| T4 + T2-2ACTB | 10.14 | 12.56 | 0.07 |
| T4 + T8-2ACTB | 10.70 | 19.35 | 0.0027 |
| T2 + T8-2ACTB | 11.56 | 12.93 | 0.38 |
| C. Composite ΔCt values by combinations of T cell phase specific markers | |||
| T4-T2 | 2.81 | 6.42 | 0.0022 |
| T8-T2 | 4.22 | 6.79 | 0.093 |
| T4-T8 | − 1.42 | − 0.37 | 0.60 |
| T4 + T8-2.T2 | 7.03 | 13.21 | 0.0006 |
ACTB: β-actin, Ct: cycle threshold of the individual target genes in RT-PCR, ΔCt: Difference of two Ct values as indicated, n: Number of samples, * P statistically significance at P < 0.05.