Fig. 5.

Conservation of the tRNA-Leu(CAG) locus between S. babjevae and S. fermentans. a) Dot matrix plot of the region containing the tRNA-Leu(CAG) locus and the neighboring protein-coding genes LYS9 and VHC1. Dots indicate regions with ≥25 matches in a 30-bp sliding window. The match at the tRNA locus is circled. b) BLASTN alignment corresponding to the region circled in a). The exons of the tRNA gene are better conserved (only 1 nt different, i.e. 98.8% identity) than the intron (77.8% identity) and the alignable region upstream of the gene (83.7% identity). Regions further upstream and downstream cannot be aligned, as shown by the dot matrix plot.