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. 2024 Jul 23;27(8):110565. doi: 10.1016/j.isci.2024.110565

Figure 4.

Figure 4

Effect of α-SYN protofibrils on mitochondria in astrocytes and U-87 MG cells

Primary astrocytes and U-87 MG were treated with 1μM α-SYN protofibrils for 3h, 6h, 12h, and 24h, and mitochondrial membrane potentials were detected using JC-1 staining (emission ratio of 527/590 nm).

(A) Primary astrocytes stained with JC-1.

(B) Quantification of green vs. red intensities, (C) Size of green/red mitochondria, and (D) mitochondria branch length by MiNA analysis were quantified from JC-1 astrocyte images.

(E) U87MG cells treated with 1μM α-SYN protofibrils for 3–24 h, were stained with MitoTracker (top panel -cyan) and JC-1 (bottom panel) to visualize mitochondrial morphology.

(F) Quantification of green vs. red intensities from JC-1 U-87 MG images. Quantification of green vs. red intensities. Quantifications are done from 10 to 15 image frames of a set, analyzing 10–20 cells from each image. Scale bars are denoted on the images. Data are expressed as mean ± SD, ∗∗∗p ≤ 0.001. Statistics were analyzed using a two-way ANOVA. n = 3.