Fig. 3.

VEGFA silencing delayed tumor formation in vivo. A172 cells stably expressing sh-VEGFA or sh-NC were injected into the left flank of the mice, and the formed tumors were harvested after 4 weeks. (A) The volume of the formed tumors was measured every 7 days for 4 cycles. (B) The effect of VEGFA knockdown on tumor weight. (C) The tumor images in the sh-VEGFA group and sh-NC group. (D) IHC assay was used to analyze Ki67 protein expression in the formed tumors in the sh-VEGFA group and sh-NC group. (E) Western blotting assay was used to detect VEGFA protein expression in the formed tumors from the sh-VEGFA group and sh-NC group. (F) TUNEL cell apoptosis detection kit was used to analyze cell apoptosis in the formed tumors from the sh-VEGFA group and sh-NC group. (G) TGF-β1 protein expression was detected by western blotting assay in the resulting tumors from A172 cells transfected with sh-NC or sh-VEGFA. ***P < 0.001