Fig. 6.

MDSCs attenuated VEGFA knockdown-induced effects by producing TGF-β1 in A172 and U251 cells. A172 and U251 cells were divided into si-NC group, si-VEGFA group, si-VEGFA + PBS group, si-VEGFA + MDSC-si-NC group and si-VEGFA + MDSC-TGF-β1 group. (A) TGF-β1 protein expression was analyzed by western blotting assay in A172 and U251 cells. (B-D) Flow cytometry assay was used to assess cell cycle and apoptosis of A172 and U251 cells. (E and F) Tube formation assay was performed to assess the effect of A172 and U251 cells on angiogenic capacity of HUVECs. (G and H) Transwell assays were used to assess the migration and invasion of A172 and U251 cells. *P < 0.05, **P < 0.01 and ***P < 0.001