Fig. 6.

Suppression of IRP2 delayed tumor growth in CRC organoids and in vivo model. (A) Cytotoxic effect of KS-20073 on CRC organoids. Organoids were treated with KS-20073 for 5 days and subjected to the cytotoxic analysis using CellTiter-Glo. (B) Representative immunofluorescence images of IRP2 (green), Phalloidin (red) and DAPI (blue) in KS-20073-sensitive organoids by treatment of KS-20073 (10 µM) were obtained using confocal microscopy. (C) IRP1, IRP2 and β-actin protein levels in KS-20073-sensitive organoids were measured using immunoblotting. (D) The protein levels of IRP1 and IRP2 were determined via immunoblotting using organoids most sensitive to KS-20073 vs. the less sensitive organoids to KS-20073. (E) Tumor volumes and images of SW480 xenografts injected intraperitoneally with vehicle or KS-20226 (100 mg/kg). Data are represented as the mean ± SEM (n = 6). **p < 0.01. (F) Tumor weights of SW480 xenografts treated with vehicle or KS-20226 (100 mg/kg). Data are represented as the mean ± SEM (n = 6). **p < 0.01. (G) Body weight after treatment with KS-20226 (100 mg/kg) for 36 days