Fig. 4.

1,25(OH)₂D₃ restored PM-mediated alterations of mitochondrial ultrastructure in BEAS-2B cells. Cells treated with PM and 1,25(OH)₂D₃ for 24 h were subjected to transmission electron microscopy (TEM) analysis and mitochondrial morphology was compared (red arrow). (A, D) Control cells showed the integrity of mitochondrial membrane and the mitochondrial cristae. (B, E) Cells exposed to PM induced impaired mitochondrial membrane integrity and disarrayed cristae were observed. (C, F) The presence of 1,25(OH)₂D₃ could oppose the impact of PM on the mitochondrial morphology and restored it. Scale bar, 1 μm