Fig. 5.

Activation of TGFβ signaling induces a switch in the ΔNp63 epigenetic interactors. (A) Heatmap depicting the intensity patterns of proteins involved in histone deacetylase binding in the respective samples. Scaled AUC was calculated using Z-score method representing quantified AUC of peptide intensities. (B-E) MCF10A MII cells, starved in 0.2% FBS medium and stimulated with TGFβ or not for the indicated time points, were subjected to immunoprecipitation (IP) with a p63-specific antibody (B-D) or an HDAC3-specific antibody (E), or IgG control, and analyzed by IB with the antibodies recognizing p63 (B-E) and DNMT1 (B), CHD4 (C), NCOR2 (D) or HDAC3 (E). In E, arrows indicate the band detecting HDAC3 expression. (F) SUZ12 interaction with p63. MCF10A MII cells, starved in 0.2% FBS medium and stimulated with TGFβ for the indicated time periods, were subjected to IP with SUZ12-specific antibody and subsequent IB with specific antibodies. In panels B-F, one of three independent experiments with similar results, is shown