Table 3.
Sialylation of decoy receptors purified from different sources
| sACE22-IgG1 proteina | Source | Neu5Acb | Neu5Gcc |
|---|---|---|---|
| sACE22.v2.4-IgG1(YTE) | Expi293F | 8.3 | Not detected |
| sACE22.S19-IgG1(YTE) | Expi293F | 7.1 | Not detected |
| sACE22.v2.4-IgG1(YTE) | CHOK1SV GS-KO | 14.4 | 0.46 |
| sACE22.S19-IgG1(YTE) | CHOK1SV GS-KO | 14.8 | 0.50 |
Measured by RP-HPLC.
Mol Neu5Ac/mol protein monomer. Limit of detection is 2.2 mol/mol protein.
Mol Neu5Gc/mol protein monomer. Limit of detection is 0.12 mol/mol protein.