Table 3.

Expression of ileal bile acid transporters after exposure of male $Ldl{r}^{-/-}$ mice to the PFAS mixture.

Ileal bile acid Transportersa	Male vehicle RNA-seq counts ($\text{mean}\pm \text{SEM}$)	Male PFAS RNA-seq counts ($\text{mean}\pm \text{SEM}$)	Overall PFAS exposure (fold change)	Overall PFAS exposure ($p$-value)	Overall PFAS exposure (FDR-adjusted $q$-value)
Slc10a1 (NTCP)	$4.7\pm 1.7$	$2\pm 0.9$	0.44	0.15	0.72
Slc10a2 (ASBT)	$\mathrm{1,771.7}\pm 136.6$	$\mathrm{2,632.9}\pm 258.9$	1.55*	$<0.001$	0.014*
Abcc2 (MRP2)	$638.2\pm 42.2$	$646.7\pm 62.4$	1.05	0.57	1.00
Abcc3 (MRP3)	$63\pm 5.1$	$78.2\pm 7.0$	1.28	0.039	0.48
Abcc4 (MRP4)	$22.2\pm 2.5$	$29.5\pm 3.9$	1.36	0.074	0.60
Abcb11 (BSEP)	$0.8\pm 0.7$	$0.7\pm 0.5$	0.85	0.89	1.00
Ephx1 (mEH)	$63.7\pm 6.2$	$57.9\pm 5.8$	0.94	0.57	1.00
Slc51$\mathit{\alpha }$ (OST$\mathrm{\alpha }$)	$\mathrm{5,279.1}\pm 319.9$	$\mathrm{6,637.5}\pm 388.3$	1.30*	$<0.001$	$<0.001$*
Slc51$\mathit{\beta }$ (OST$\mathrm{\beta }$)	$\mathrm{2,300.2}\pm 175.2$	$\mathrm{3,212.6}\pm 242.2$	1.47*	$<0.001$	$<0.001$*

Note: Statistical significance was determined by $t$-test using R statistical software (version 4.2.0; R Developmental Core Team). FDR, false discovery rate; Ldlr, low density lipoprotein receptor; PFAS, per- and polyfluoroalkyl substances; RNA-seq, RNA sequencing; SEM, standard error of the mean. *Represents significant change relative to vehicle control ($q<0.05$).

^a $N=20$ total; $n=10$ male vehicle and $n=10$ male PFAS.