Figure 5. Microglial interaction with AgRP axon terminals in the PVH and ARH.

(A-D) Maximum projection images of microglial cells (green) labeled AgRP terminals (red), and CD68 (lysosomal associated membrane protein and phagocytic capacity marker, pink). (E-H) Digital 3D reconstructions shown in (A-D) after application of a digital zoom to more clearly illustrate engulfment of labeled AgRP terminals by microglia (I-L). (M-P) Graphical comparisons between groups to illustrate the effects of age and MHFD exposure on CD68 expression and AgRP terminal engulfment. Bars represent the mean ± SEM and each point represents one animal. *P<.05, **P<.005. Abbreviations: AgRP, agouti-related peptide; ARH, arcuate nucleus or the hypothalamus; CD68, Cluster of Differentiation 68; MHFD, maternal high fat diet during lactation; PVH, paraventricular nucleus of the hypothalamus.