Figure 5.

A) PMF of two-site symmetric system with symmetric 100 mM concentrations. B) Ionic flux through channel under different conditions: +140 mV electronic potential (blue), the Nernstian equivalent chemical concentration gradient [199.15 mM] ext : [0.85 mM] int (yellow), and a +140 mV electronic potential combined with a [0.85 mM] ext : [199.15 mM] int chemical gradient (Nernst equilibrium, green). C) Populations for conditions in (B). D) PMF of Nernst equilibrium at −140 mV (blue) and +140 mV (red), highlighting unique equilibrium populations and barrier heights.