Table 3.
Different concentrations of ADEE used for the MIC determination.
| Tube number/label | Concentration (μg/mL) of compound | Observed result | |
|---|---|---|---|
| Antibiotic (tetracycline) | ADEE | ||
| 1 | 10 μg/mL | MIC | MIC |
| 2 | 5 | MIC | MIC |
| 3 | 2.5 | MIC | MIC |
| 4 | 1.25 | MIC | MIC |
| 5 | 0.625 | MIC | MIC |
| 6 | 0.3125 | MIC | Growth |
| 7 | 0.15625 | Growth | Growth |
| 8 | 0.078125 | Growth | Growth |
| 9 | 0.0390625 | Growth | Growth |
| 10 | 0.01953125 | Growth | Growth |
| 11-Positive control | NIL | Growth | Growth |
| 12-Blank/sterility control | NIL | — | — |
The value 0.625 represents the minimum inhibitory concentration (MIC) of the antibiotic tetracycline, indicating the lowest concentration (10 μg/mL) of the aqueous extract of ADEE at which tetracycline successfully inhibited bacterial growth. This MIC value highlights tetracycline's potency against the tested bacteria, even at a relatively low concentration when combined with ADEE.