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. 2024 Mar 21;96(2):494–501. doi: 10.1038/s41390-024-03135-1

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© The Author(s) 2024

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Fig. 4 — a Plasmids MS-0 and MS-100 were mixed to prepare MS-25, MS-50 and MS-75 in varying proportions. b The sequence of the loci adjacent to the IFI44L methylation site and the primers used for HRM-qPCR amplification. c Illustrative examples showcasing both positive and negative results within the system.