Fig. 4. MEF2B interacts with the SWI/SNF complex.

a Detection of MEF2B/SMARCA4 interaction by MEF2B immunoprecipitation (IP) followed by immunoblotting (IB) using nuclear extracts of SUDHL10 cells or normal GC B cells isolated from human tonsil. Lamin B1 was used as a nuclear fraction control. Normal GC B cell samples are identical to those used in Fig. 2e. The experiment was repeated twice. b Heat maps showing the read density and distance plots displaying the distribution of MEF2B or SMARCA4 bound regions in relationship to each other closest peak, as detected in normal GC B cells by Cut&Run-seq analysis. In the heat maps, data is normalized to number of reads per bin/sum of all reads per bin (in millions). c Genomic binding profile of MEF2B and SMARCA4 at BCL6, CCND3, CXCR4, CD83, CD86 and CD79A loci, as detected by Cut&Run-seq analysis in normal GC B cells. Significantly bound regions that were recurrently detected in both biological replicates (2 independent donors) are displayed as bars under the read profiles. MEF2B/SMARCA4 overlapping bound regions are displayed as black bars at the bottom (MEF2B/SMARCA4 overlap). Genomic coordinates based on hg38. BPM, bins per million map reads. Source data are provided as a Source Data file.