Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Aug 23;15:7269. doi: 10.1038/s41467-024-51736-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

PMC Copyright notice

Fig. 6 — a Binding peaks of STAT3 around indicated gene region. b mRNA expression of the reparative markers Cd163, Mrc1, Tgfb1, Pdgfb and Vegfb in PPARγ^+/+ and PPARγ^A/A BMDMs treated with STAT3 inhibitor Stattic (5 μM). n = 3 biological replicates per group. c Representative flow cytometry histograms and quantifications of surface levels of CD163 and CD206 in PPARγ^+/+ and PPARγ^A/A BMDMs treated with stattic for 48 h (n = 3 biological replicates per group). d The protein expression of phosphorylated (Tyr705) STAT3 and total STAT3 in PPARγ^+/+ and PPARγ^A/A BMDMs in response to free fatty acid (FFA). β-Actin was used as a control. e Transcriptional assays of fatty acid on STAT3-driven gene transcription in dual-luciferase reporter assay. Vegfb, Pgdfb and Tgfb1 promoter was transfected in HEK293T cells with or without STAT3 plasmid in response to FFA. n = 4 biological replicates per group. f CUT&RUN qPCR of STAT3 binding to the indicated promoters in BMDMs treated with C75 in response to IL-10 (n = 3 biological replicates per group). Data were normalized to the spike in DNA. g CUT&RUN qPCR of STAT3 binding to the indicated promoters in BMDMs treated with oleate or palmitate (n = 3 biological replicates per group). Data were normalized to the spike in DNA. Unless specified otherwise, the data are presented as means ± s.e.m. (error bar) and compared using the two-tailed Student’s t test. Source data are provided as a Source Data file.