Fig. 6.

Semi-preparative HPLC–UV-chromatograms after SPE processing. Apicidin F (1) eluting at 15.4 min and apicidin J (2) eluding at 13.7 min were purified from combined SPE fractions, while apicidin K (3) eluting at 15.2 min and apicidin L (4) eluting at 13.6 min were separated successfully on the SPE and processed individually. Chromatographic separation was achieved on a ReprosilPur 120 C₁₈-AQ column (250 × 10 mm, Dr. Maisch GmbH) equipped with a C₁₈ guard column (4 × 3 mm, Phenomenex) and recorded at λ = 225 nm