Fig. 9.

Cytotoxicity of the isolated apicidins (1–4) and apicidin (5) via resazurin assay in HepG2 (a, left) and A549 (b, right) after 24 h of substance incubation normalized to the negative control (neg, 1% DMSO). Applied substance concentration ranged from 0.01 to 100 µM. Positive control (pos) was 10 µM T2-toxin. Black shows apicidin F (1), dark grey shows apicidin J (2), grey shows apicidin K (3), light grey shows apicidin L (4), and white shows apicidin (5). Experiments were conducted with three individual cell lines with three biological replicates each (N = 9). Columns depict mean values ± standard deviations in %. Statistically significance was tested by one-way ANOVA and post hoc Tukey’s test: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001