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. 2024 Aug 23;7:1040. doi: 10.1038/s42003-024-06715-3

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 1 — A zebrafish otoliths indicated by white arrows at 72 hpf. B Quantification and t-test of zebrafish otoliths from genotyped mutants characterized as separate or fused otoliths. t-test, n = 75. C Alcian blue representation of a 6 dpf zebrafish wildtype and esrp1^−/− esrp2^−/− double mutant showing cartilage stain, yellow line shows the measurement of the distance between the midline of Meckel’s and ceratohyal cartilages. D quantification and t-test analysis of this measurement in wildtype (n = 11) and esrp1^−/−; esrp2 ^−/− mutants (n = 14). E Alcian blue and Alizarin red staining of larvae at 7 dpf ventral view, the pharyngeal teeth are present in wildtype (white arrows). In contrast, the esrp1^−/−; esrp2^−/− all exhibit decreased number of teeth, and occasionally some double mutants lack all ceratobranchial cartilages and the pharyngeal teeth are absent. F wildtype and esrp1^−/− esrp2^−/− mutant pectoral fins labeled with sox10 mCherry (red) and krt4 gfp (green).