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. 2024 Aug 23;15:7272. doi: 10.1038/s41467-024-51410-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 3 — a Each bar represents an m⁵C site mapped to the 16S-tRNA^Ala-23S operon; bar height represents modification frequency and its color corresponds to the enzyme responsible for its installation. One m⁵C site was mapped antisense to the 16S rRNA (black outline). Additional information is provided in Supplementary Data 5. b, c m⁵C sites (red spheres) are not randomly distributed. 16S subunit (blue), 23S subunit (nude), 5S subunit (orange), A, P, and E site tRNAs (green), and the mRNA (pink) are shown at 3 angles rotated at 90°.