Figure-1.

Microscopic examination of post-thawed frozen semen. (a) Average post-thawing spermatozoa viability for each treatment group, as determined using the analysis of variance (ANOVA) test which shows (***) = p < 0.0001. (b) Average post-thawing spermatozoa motility for each treatment group, as determined using the ANOVA test which shows (***) = p < 0.0001. (c) Average post-thawing spermatozoa membrane integrity for each treatment group using the Kruskal–Wallis test, which indicated (**) = p < 0.03. (d) Image of (a) live and (b) dead sperm on eosin-nigrosin staining. (e) Image of (a) a swollen tail and (b) no swollen tail by HOS test. P0 (CoQ10 0 mg/dL), P1 (CoQ10 6.25 mg/dL), P2 (CoQ10 12.5 mg/dL), P3 (CoQ10 25 mg/dL), P4 (CoQ10 50 mg /dL), and P5 (CoQ10 100 mg/dL). ANOVA=Analysis of variance.