Table-2.

Series of pair of primers for cycling of E2 open reading frame.

Primer	Sequence 5′–3′	Position	BVDV gene E2 (at nucleotide level) length obtained through MEGA-11	Position of coding’s region of E2 gene	Size of E2 gene (at amino acid level) length obtained through MEGA-11	Target BVDV gene size after gel electro- phoresis	Reference
E2_fwd P7_rev	TGGTGGCCTTATGAGAC CCCATCATCA CTATTTCACC	2345– 3356	1011 bp	2881– 3218	337 bp	E2 gene 1464 bp	[45]

BVDV=Bovine viral diarrhea virus