Fig.1.

The effect of AST, MTX and AST+MTX on viability of NALM-6 cells by MTT assay. Cells were treated with different doses of AST, MTX and AST+MTX during a specific incubation period. The IC50 dose of AST and MTX was 25μM and 300nmol, respectively (A, B). The combination therapy of MTX and AST resulted in a substantial reduction in the necessary dosage of MTX to just one-thirtieth (10nmol). (C) There was also a notable decline in NALM-6 cell viability when compared to HDF cells. HDF cells treated with the IC50 value of different treatments which were used as the control group (G) (P<0.0001).