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. 2024 Aug 12;15:1360587. doi: 10.3389/fphar.2024.1360587

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Copyright © 2024 Liu, Zhao, Liu, Wu, Li, Fu, Yang and Liang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Shikonin treatment can inhibit colon cancer cell growth in a dose-dependent way. (A) Chemical structural formula of shikonin. (B–D) CCK-8 assay detecting the cell viabilities and IC₅₀ values of HT29 and HCT116 cell lines. (E,F) Images of shikonin treatment dose-dependently inhibiting the growth of HT29 and HCT116 cells. Data are shown as mean ± S.E.M. **p < 0.001; ****p < 0.0001. Each experiment was performed in triplicate and repeated three times.