supplemental Fig. S1.

Effects of S1P turnover on cell viability and lipid droplet formation in INS1E beta-cells after exposure to low concentrations of FFA. INS1E cells were incubated in the absence or presence of PA and/or OA (low-dose: 250 μM of each, high dose and mixtures: 500 μM) for 24 h. Thereafter, (A) cell viability was measured by MTT assay, shown are Means ± SEM from n = 3–4 independent experiments, each conditions was measured in triplicates. ANOVA followed by Bonferroni, ∗P < 0.05 versus untreated. (B) lipid droplets (after exposure to 250 μM OA) were visualized by OilRed staining, shown are representative pictures of n = 2 individual experiments. Bars: 20 μm.